4-MU4-deoxy-xyloside reduced TGF-β1-induced increased expression and actvation of endogenous TGF-β1.
(A) Lung fibroblasts were treated with 500 μM of 4-MU4-deoxy-xyloside and/or TGF-β1 (5 ng/ml) for 6 h and the expression of endogenous TGF-β1was analyzed by qPCR. The relative expression was normalized with ribosomal protein S29. Data are expressed as mean ± SD of three separate experiments, (*P<0.05). (B) Fibroblasts were treated with 500 μM of 4-MU4-deoxy-xyloside and/or TGF-β1 (5 ng/ml) for 24 h and the total and active TGF-β1 levels were measured by ELISA in cell culture supernatants as described in Materials and methods section.