2D-DIGE image of proteins of SDS- (A) or sarkosyl-insoluble (B) aggregates isolated from the BY4742 [PIN+] strain expressing Aβ-GFP and from its [pin−] derivative expressing non-fused GFP.
Spots corresponding to proteins from [PIN+] cells expressing Aβ-GFP are red, while proteins from GFP-containing cells (control) are green. Yellow spots correspond to proteins present in both compared samples. Proteins identified by mass-spectrometry are indicated. Identification data are presented in Table 1 (SDS-insoluble aggregates) and 2 and 3(sarkosyl-insoluble aggregates). Mass-spectra of identified proteins are listed in S1–S3 Tables, correspondingly.