Western blot analysis on purified rKer eluted from the Ni2+-NTA affinity chromatography.
2017-08-23T17:59:29Z (GMT) by
Lane 1, positive protein with Flag tag; Lane 2, cell lysates; Lane 3, cell supernatants and lane 4, purity. Signals of western blot were detected by a monoclonal antibody against C-terminus of Flag tag.
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