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Western blot analysis on purified rKer eluted from the Ni2+-NTA affinity chromatography.

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posted on 2017-08-23, 17:59 authored by Miaorong Huang, Ruiai Chen, Guangcai Ren

Lane 1, positive protein with Flag tag; Lane 2, cell lysates; Lane 3, cell supernatants and lane 4, purity. Signals of western blot were detected by a monoclonal antibody against C-terminus of Flag tag.

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    Bacillus licheniformis keratinasepeptidesequence encoding Ker-His-Flagcoli DH 10Bac cellinsect cells systempUC 57 vectorexpressionBacillus licheniformis PWDDNAsodium dodecyl sulfate polyacrylamide gel electrophoresisNTA affinity chromatographyprotein8 His-Flag taginsect cellsinsect Spodoptera frugiperdaSDS-PAGE

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