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WNT enhances and NODAL inhibits BMP signaling at colony edge.

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posted on 2019-10-15, 17:39 authored by Sapna Chhabra, Lizhong Liu, Ryan Goh, Xiangyu Kong, Aryeh Warmflash

Images of samples immunostained for the indicated markers after 44 h of treatment in different conditions: control (wild-type) hESCs and NODAL−/− cells were treated with 50 ng/ml BMP4. The sample labelled as IWP2 at 0 h represents wild-type cells treated with 50 ng/ml BMP and 5 μM IWP2. Quantification represents intensity levels of indicated markers normalized to DAPI, averaged at different positions along the colony radii. Error bars represent standard error of the mean. N ≥ 10. Scale bar = 100 μm. Underlying data can be found in S7 Data. BMP, Bone Morphogenic Protein; hESC, human embryonic stem cell.

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