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WB analyses of synaptophysin in the cortex of TG and WT mice 9 months post-irradiation.

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posted on 2017-11-29, 18:23 authored by Emil Rudobeck, John A. Bellone, Attila Szücs, Kristine Bonnick, Shalini Mehrotra-Carter, Jerome Badaut, Gregory A. Nelson, Richard E. Hartman, Roman Vlkolinský

The average synaptophysin levels in TG control mice were significantly elevated when compared to WT controls. Irradiation of TG mice at 0.5 and 1 Gy had no effect on synaptophysin levels. In WT mice, irradiation significantly elevated synaptophysin to levels comparable to those observed in TG groups. Insets: Original images of WB gels prepared from TG (top panel) and WT (bottom panel) mice. Immuno-reactive bands optical densities indicate levels of synaptophysin (~38 kDa) and β-tubulin (~50 kDa) in cortical homogenates of control and irradiated 0.5 Gy mice. TG mice: N = 6, 5, 5 animals/radiation group; WT mice: N = 8, 8 animals/radiation group. Statistical analyses: 2-way ANOVA, Post hoc *** p<0.001 WT 0 Gy vs 0.5 Gy; †† p<0.01 WT vs TG controls. Data represent means ± SEM.

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