pbio.3000688.g003.tif (5.03 MB)

UA promotes thermogenesis in BAT and induces browning of iWAT.

figure

posted on 2020-03-27, 17:34 authored by Bo Xia, Xiao Chen Shi, Bao Cai Xie, Meng Qing Zhu, Yan Chen, Xin Yi Chu, Guo He Cai, Min Liu, Shi Zhen Yang, Grant A. Mitchell, Wei Jun Pang, Jiang Wei Wu

(A) Tissue weights of BAT, iWAT, and eWAT in UA-treated mice and controls for a period of 10 weeks under HFD feeding (n = 6). (B) Morphological changes in the 3 depots of fat shown by HE staining. (C) Immunohistochemistry staining of UCP-1 in sections of 3 fat depots. (D) mtDNA copy number in BAT, iWAT, and eWAT (n = 6). (E) mRNA levels of thermogenic genes in BAT, iWAT, and eWAT (n = 6). (F) Immunoblot analysis of UCP-1 in BAT, iWAT, and eWAT. (G) Immunofluorescence and (H) immunoblot analysis of UCP-1 in primary cultured adipocytes from iWAT and BAT. (I) mRNA levels of thermogenic genes in primary cultured adipocytes from iWAT and BAT of UA-treated mice and controls (n = 6). (J) OCR measured by a Seahorse analyzer in brown adipocytes and beige adipocytes treated with or without UA. The underlying data for this figure can be found in S1 Data. AA, antimycin A; BAT, brown adipose tissue; Cidea, Cell death inducing DFFA like effector a; Cox7a, Cytochrome c oxidase subunit 7a1; Cpt1b, Carnitine palmitoyltransferase 1B; Cyt c, Cytochrome c; Dio2, Deiodinase 2; Errα, Estrogen related receptor α; eWAT, epididymal WAT; FCCP, carbonyl cyanide-4-(trifluoromethoxy) phenylhydrazone; HE, hematoxylin–eosin; HFD, high-fat diet; iWAT, inguinal WAT; mtDNA, mitochondrial DNA; nuDNA, nuclear DNA; OCR, oxygen consumption rate; Pgc1-α, Peroxisome proliferator-activated receptor gamma coactivator 1α; UA, urolithin A; UCP-1, Uncoupling protein 1; WAT, white adipose tissue.