UA-mediated thermogenesis and body weight control in mice is preserved at thermoneutrality.

(A) Schematic diagram of mice treatment. (B) Body weight of HFD-fed mice treated with UA or vehicle at 30°C (n = 6). (C) Daily food intake of mice at 30°C (n = 6). (D) Body composition measured by DEXA scan after 6 weeks of treatment. (E) Oxygen consumption of mice tested for 24 h (n = 6). (F) The body temperature of mice at 30°C (n = 6). (G) Representative infrared images of the vehicle and UA-treated mice. mRNA levels of thermogenic genes in (H) iWAT and (I) BAT from UA-treated or vehicle control mice at 30°C (n = 6). (J) NE-stimulated whole-body oxygen consumption in UA-treated mice and controls mice at 30°C. (K) mRNA levels of thermogenic genes in denervated BAT of UA-treated or vehicle mice at room temperature. The underlying data for this figure can be found in S1 Data. BAT, brown adipose tissue; Cox7a, Cytochrome c oxidase subunit 7a1; DEXA, dual-energy X-ray absorptiometry; Dio2, Deiodinase 2; HFD, high-fat diet; iWAT, inguinal white adipose tissue; NE, norepinephrine; Pgc1-α, Peroxisome proliferator-activated receptor gamma coactivator 1α; UA, urolithin A; Ucp1, Uncoupling protein 1.