Travelled distance of different markers in strains with WT or rearranged configurations.
Columns indicate the mean value with standard deviation calculated for 30 independent foci. The x axis represents the chromosome genetic map (position in Mb from thrABC operon). The MDs (Ori and Right) and the NSRight region are indicated above the graph: the transposed segments are indicated by a line below the WT map, the insertion point by arrowhead. (A) WT strain and its derivative upon transposition of segment ori Td-1 (coordinates 3928826–4067141; strain OriT1inRight17) in the Right MD (attB17). Markers Ori-6, Ori-7, Ori-3, Ori-4, NSR-5 and Right-5 are indicated. (B) WT strain and its derivatives upon transposition of the 318-kb long segment ori Td-4 (coordinates 4379216–55668; strain OriT4inRight17) and NSR Td-1 (strain NSRT1inRight17) in the Right MD (attB17), respectively. Markers Ori-5, Ori-3, Ori-4, NSR-1, NSR-2, NSR-5, Right-2 and Right-5 are indicated. (C) WT strain and its derivative upon transposition of 264-kb segment NSR Td-2 (coordinates 66848 to 331520; strain NSRT2inOri87.6) in the Ori MD (87.6 min). Markers Ori-3, Ori-4, NSR-1, NSR-2 and NSR-5 are indicated. (D) WT strain and its derivatives upon transposition of 154-kb segment right Td-1 (coordinates 649460–806549) at two different positions, 92.7 min (position 4302204; strain RightT1in Ori92.7) and 87.6 min (position 4067141; strain RightT1inOri87.7), respectively. Markers Ori-1, Ori-3, Ori-4, Right-2 and Right-5 are indicated.