Transcriptome analysis of XX, XO and XY oogenesis in vitro.

(A) PCA analysis of ESCs, EpiLCs, PGCLCs and PGCLCs/oocytes in agg-3-7. Each dot represents the data compiled from three independent experiments, except in the case of ESCs, where the dots represent the data from two independent experiments. (B) Heatmap analysis of the transcriptome of agg-7. The heatmap and clustering are based on the average of the transcripts per million (TPM) gene expression levels. The number of DEGs and representative genes are shown at the right of the heatmap. (C) PCA analysis of PGCLCs/oocytes in agg-9-13. Each dot represents the data compiled from two independent experiments. (D) Heatmap analysis of the transcriptomes of agg-9 and -11. The heatmap and clustering are based on the average of the transcripts per million (TPM) gene expression levels. The number of DEGs and representative genes are shown at the right of the heatmap. (E) Immunostaining of active Caspase 3. The graph depicts the results of the immunostaining analysis. (F) Immunostaining of cleaved PARP1. The graph depicts the results of the immunostaining analysis. Scale bars, 10 μm. The data was compiled from at least three independent experiments. P values were calculated by Tukey’s HSD test. ***P<0.001, *P<0.05; NS, not significant.