Transcriptionally active and differentially regulated genes in B. pseudomallei 1026b in vivo.

Transcriptionally active open reading frames were categorized into different metabolic function groups based on cluster of orthologous groups (COG) annotation assignments and distribution of hypothetical and annotated genes indicated on each chromosome. Transcriptionally active genes during in vitro and in vivo treatment (A) and distribution of annotated and hypothetical genes encoded on each chromosome (B). Unique transcriptionally active genes in vitro (C) and distribution of annotated and hypothetical genes encoded on each chromosome (D). Differentially regulated genes during in vitro treatment (E) and distribution of annotated and hypothetical genes encoded on each chromosome (F). Unique transcriptionally active genes during in vivo growth (G) and distribution of annotated and hypothetical genes encoded on each chromosome (H). Differentially regulated genes during in vivo treatment (I) and distribution of annotated and hypothetical genes encoded on each chromosome (J). COG groups: D, cell division and chromosome partitioning; M, cell envelope biogenesis/outer membrane; N, cell motility and secretion; O, posttranslational modification/protein turnover/chaperons; T, signal transduction mechanisms; U, intracellular trafficking and secretion; V, defense mechanisms; J, translation/ribosomal structure and biogenesis; K, transcription; L, DNA replication/recombination/repair; E, amino acid transport and metabolism; C, energy production and conversion; F, nucleotide transport and metabolism; G, carbohydrate transport and metabolism; H, coenzyme metabolism; I, lipid metabolism; P, inorganic ion transport and metabolism; Q, secondary metabolites biosynthesis/transport and catabolism; Hyp, and poorly characterized.