Tomato pulp and peel total allergenicity in IL7-3, IL12-4 and in the cultivated line M82 assessed by slot blotting.
(A) Example of a X-ray film obtained by slot blot analyses using pooled sera of allergic patients; three different replicates for each line were analysed. (B) Assessment of total allergenicity, through image analysis, measuring integrated optical density (IOD) of immunoreactive bands. Different letters indicate significant differences among the samples (ANOVA and Tukey’s test, p < 0.05). See text for details.