pone.0214737.g005.tif (3.74 MB)
Thrombin cleaves S3ED from human lung microvascular endothelial cells.
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posted on 2019-05-15, 17:25 authored by Melanie Jannaway, Xiaoyuan Yang, Jamie E. Meegan, Danielle C. Coleman, Sarah Y. YuanImmunofluorescence was used to verify expression of syndecan ectodomains on human lung microvascular endothelial cells (HLMVECs). All four syndecans were found expressed on HLMVECs, with thrombin treatment (1 hour) significantly decreasing the expression of S3ED and S4ED (p<0.05).
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thrombin receptor PAR 1.barrier integritydisease statesbarrier functionsyndecan -3 ectodomain fragmentsSyndecan proteinsplasma protein leakageglycocalyxRho kinase inhibitorELISAVE-cadherin-based adherens junction disorganizationalbumin-bound Evanstransmission electron microscopysyndecan subtypesF-actin stress fibersmouse lungintercellular gapstransmigrating cellsbarrier dysfunction Objectiveparacellular hyperpermeabilitythrombotic disease statescleavage productsparacellular permeabilityblot analysislung microvasculature
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