Thrombin-induced changes in syndecan ectodomain proteins from ex vivo human lung.

ELISA was performed to measure the concentration of each of the syndecan ectodomains in the supernatant of fresh human lung slices treated with vehicle or thrombin. Thrombin treatment (10 U/ml; 24 hours) of ex vivo human lung generated no difference in concentration of human (A) S1ED (n = 4, p = 0.47) or (B) S2ED (n = 4, p = 0.13). However, the concentrations of both (C) S3ED (n = 5, p = 0.0072) and (D) S4ED (n = 5, p = 0.006) were significantly decreased following thrombin treatment.