The threshold for nociceptive response measured by calibrated forceps was stable for 5 days.

(A) Time courses of the nociceptive response threshold of 6 mice over 5 days. A repeated-measured one-way ANOVA revealed that there was no significant difference among days (F4,20 = 0.3240, p = 0.8585). (B) The thresholds were replotted as the mean ± SEM for 6 animals for each day.