The subcellular localizations of mCherry-Ubc13 and Rad5-sfGFP.

HK578-10A (upper A) and HK578-10D (upper B) are the wild-type yeast strains without modification; the tagged strains PPGK1-Ubc13-mCherry and PPGK1-Rad5-sfGFP are shown at the bottom of A and bottom of B, respectively. The nucleus was stained by DAPI. The images were obtained under Plan-Apochromat 63×/1.40 oil (Zeiss LSM780).