pgen.1008585.g004.tif (1.57 MB)
The structure of N-DRC is disrupted in Drc7 KO spermatozoa.
figure
posted on 2020-01-21, 18:34 authored by Akane Morohoshi, Haruhiko Miyata, Keisuke Shimada, Kaori Nozawa, Takafumi Matsumura, Ryuji Yanase, Kogiku Shiba, Kazuo Inaba, Masahito Ikawa(A) Presence of other N-DRC components in spermatozoa. Drc7 KO spermatozoa had decreased levels of DRC3, GAS8 and TCTE1. In contrast, the signal of RSPH6A and RSPH9 were detected at comparable levels. (B) Immunostaining of DRC3 (left) and GAS8 (right) in spermatozoa. Enlarged pictures of Drc7 KO spermatozoa are shown in right panel. Hoechst (blue), nucleus; acetylated-tubulin (green); DRC3 or GAS8 (red). Scale bar in upper, 50 μm, below, 10 μm. (C) Signal intensity ratio of DRC3/acetylated tubulin (upper) and GAS8/acetylated tubulin (lower) shown in (B). The signal of DRC3 and GAS8 were significantly weaker in Drc7 KO spermatozoa. Error bars represent S.D. ***p < 0.001 (unpaired Student's t-test).
