The roles of FZD6 in cell viability of siRNA treated cells.

(A) CCK-8 results of IPEC-J2 cells at different time points. NC = Negative Control; Data are expressed as the mean ±SEM, n = 6. (B) Flow cytometric analysis of Annexin V/PI in IPEC-J2 cells. IPEC-J2 cells were treated with 30nm, 50nm and 100nm FZD6 siRNA-1 for 48h, respectively. Untreated cells were considered a negative control. (C) Relative apoptosis rate of cells in each group; Data are expressed as the mean ±SEM, n = 3. Values not sharing common letters are significantly different at P < 0.05.