The original bNK2A-C10C20 and an analog bNK2A-S10S20 peptides showed similar α-helical confirmations.

Circular dichroism (CD) spectral analysis of the peptides (20 μM final concentrations) was performed at room temperature using Jasco J-850 CD spectrophotometer in the presence of or absence of 50% TFE. Measurements were taken every 0.1 nm from 200 nm to 250 nm. Shown graphs are the means of six accumulations after baseline correction for each peptide.