pgen.1008508.g002.tif (1.02 MB)

The cdr-1p::gfp transcriptional reporter is induced by 100μM cadmium and 200μM zinc in cdk-8– and mdt-15–dependent fashion.

figure

posted on 2019-12-09, 18:31 authored by Naomi Shomer, Alexandre Zacharie Kadhim, Jennifer Margaret Grants, Xuanjin Cheng, Deema Alhusari, Forum Bhanshali, Amy Fong-Yuk Poon, Michelle Ying Ya Lee, Anik Muhuri, Jung In Park, James Shih, Dongyeop Lee, Seung-Jae V. Lee, Francis Christopher Lynn, Stefan Taubert

[A] Illustration of the cdr-1p::gfp reporter and putative regulatory DNA elements in the cdr-1 promoter; note, the diagram is not to scale. For details, see text. [B, C] Representative micrographs of worms bearing the cdr-1p::gfp and myo-2p::mCherry (transgenic marker) transcriptional reporters, without and with exposure to [A] 100μM cadmium and [B] 200μM zinc. [D] The graphs show the average fluorescence intensity (arbitrary units, A.U.) of worms bearing the cdr-1p::gfp transcriptional reporter. The two panels on the left show WT worms bearing the cdr-1p::gfp transcriptional reporter fed either control or mdt-15 RNAi and exposed for four hours to 0μM or 100μM Cd or 0μM or 200μM Zn (control RNAi 0μM Cd: n = 32 worms total from 3 independent repeats; control RNAi 100μM Cd: n = 31 worms total from 3 independent repeats: mdt-15 RNAi 0μM Cd: n = 19 worms total from 3 independent repeats; mdt-15 RNAi 100μM Cd: n = 32 worms total from 3 independent repeats; control RNAi 0μM Zn: n = 30 worms 3 total from independent repeats; control RNAi 200μM Zn; n = 29 worms total from 3 independent repeats; mdt-15 RNAi 0μM Zn: n = 42 worms total from 3 independent repeats: mdt-15 RNAi 200μM Zn: n = 33 worms total from 3 independent repeats). The two panels on the right show WT and cdk-8(tm1238) worms exposed for four hours to 0μM or 100μM cadmium (Cd) or 0μM or 200μM zinc (Zn) (WT 0μM Cd: n = 39 worms total from 3 independent repeats; WT 100μM Cd: n = 39 worms total from 3 independent repeats; cdk-8(tm1238) 0μM Cd: n = 29 worms total from 3 independent repeats; cdk-8(tm1238) 100μM Cd: n = 38 worms total from 3 independent repeats; WT 0μM Zn: n = 31 worms total from 3 independent repeats; WT 200μM Zn: n = 32 worms total from 3 independent repeats; cdk-8(tm1238) 0μM Zn: n = 31 worms total from 3 independent repeats; cdk-8(tm1238) 200μM zinc: n = 24 worms total from 3 independent repeats). Error bars: standard deviation. Statistical analysis: **** p < 0.0001, n.s. = not significant, two-way ANOVA, multiple comparisons, Tukey correction; comparisons are to WT or control(RNAi) unless indicated by lines.