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The effects of ClpP and ClpS/ClpA on P. aeruginosa virulence.

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posted on 2016-04-27, 10:59 authored by Jingru Zhao, Xiang Yu, Miao Zhu, Huaping Kang, Jinbiao Ma, Min Wu, Jianhua Gan, Xin Deng, Haihua Liang

(A and B) The production of pyocyanin was evaluated in wild-type PAO1, ΔclpSΔclpA, ΔclpP, ΔclpSΔclpAΔcdpR, ΔclpPΔcdpR, and their complemented strains. **p < 0.05 based on Student’s t test. EV represents empty vector. (C) The expression of phzA1-lux in wild-type PAO1, ΔclpSΔclpA, ΔclpP, ΔclpPΔcdpR and ΔclpSΔclpAΔcdpR strains. Results represent means ± SD, and data are representative of two independent experiments. (D) Deletion of clpP or clpS/clpA in P. aeruginosa decreased the pathogenicity in a mouse model. C57BL6 mice were intranasally challenged with wild-type PAO1, ΔclpP, ΔclpSΔclpA, and the complemented strains (ΔclpP/CTX-clpP, ΔclpSΔclpA/CTX-clpSclpA) at 1x107 cfu in 50 μL PBS, and moribund mice were killed to obtain survival data (Kaplan-Meier Curve with Log-Rank test, p = 0.0043 (ΔclpP), p = 0.0028 (ΔclpSΔclpA), n = 6).

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