pone.0216711.g001.tif (233.76 kB)
The effect of H2O2 on the viability of RAW264.7 cells.
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posted on 2019-05-21, 17:32 authored by Xinyu Lin, Dingping Bai, Zixi Wei, Ying Zhang, Yifan Huang, Hui Deng, Xiaohong HuangCells were treated with different concentrations of H2O2 (0, 100, 200, 400, 600, 800, 1000 μM) for 24 h. The results are expressed as the mean±SE of six independent experiments. *P < 0.05 and **P < 0.01 compared with H2O2-untreated cells.
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GCLCNrf 2-Keapmiddle-dose curcumin-treated groupsNrf 2-Keap pathway Large-scaleCurcumin attenuates oxidative stresshigh-dose curcumin-treated groupsmiddle-dose curcumin upregulated Nrf 2 expressionRAW 264.7 cellsNrf 2 proteinNrf 2-Keap pathwayWestern blot analysisH 2 O 2 treatmentROSmiddle-dose curcuminreactive oxygen speciesMDAGLCMapoptosioxidative stressH 2 O 2HOmiddle-dose curcumin-treated groupSODglutamate-cysteine ligaseGSH-PXNrf 2MTTantioxidant enzymes
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