The changes in protein gene product (PGP) 9.5 immunoreactivity and the expression of PGP 9.5 mRNA of rat esophagus with aging.

Representative images of PGP 9.5-immunoreactive cells in myenteric plexus (x1000 magnification) (A), PGP 9.5 immunohistochemistry expressed as percentage of PGP 9.5-immunoreactive cells per total cells (B), PGP 9.5 immunohistochemistry expressed as percentage of PGP 9.5-immunoreactive areas per total areas (C), the expression of PGP 9.5 mRNA measured by real time-PCR (D) in 6-, 31–74-wk-old rats (each, n = 7) and 2-yr-old rats (n = 10). Each bar represents the mean ± standard error.