pone.0230915.g004.tif (828.32 kB)
TLC analysis of bioconversion products of N. cholesterolicum NRRL 5767 transformants.
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posted on 2020-03-27, 20:05 authored by Jenq-Kuen Huang, Kadidia Samassekou, Hekmat B. Alhmadi, David R. VanDerway, Joshua D. Diaz, Jacob A. Seiver, Shawn W. McClenahan, Scott M. Holt, Lisa Wen(A) Lane 8 contains fatty acid standard. Lanes 1–7 and 9–16 are individual transformants with OA as substrate. (B) Lanes 1, 8, and 13 contain fatty acid standard. Lanes 2 and 3 are N. cholesterolicum NRRL5767 with and without OA, respectively. Lanes 4 and 5 are transformant 1-3-3 with and without OA, respectively. Lanes 6 and 7 are knockout mutant 1-3-17 with and without OA, respectively. Lanes 9 and 10 are knockout mutant 2-3-52 with and without OA, respectively. Lanes 11 and 12 are clone 1-p-11 with and without OA, respectively.
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Nocardia cholesterolicum NRRL 5767. Existencealcohol dehydrogenase geneknockout mutants offer improvementsoleate hydratase isozymesNocardia cholesterolicum NRRL 5767Nocardia cholesterolicum NRRL 5767DNAGolden Gate Assemblyalcohol dehydrogenase activityNocardia cholesterolicum NRRL 5767 strainalcohol dehydrogenase knockout mutants10- hydroxystearic acidoleic acid10- ketostearic acidCRISPRalcohol dehydrogenase
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