T168 and T181 mutants show normal subcellular LIN-5 localization.

Immunohistochemical staining of embryos heterozygous for egfp::lin-5 and wild type or phosphomutant lin-5 as indicated. L4 animals were treated with feeding RNAi against control or egfp RNAi to specifically remove egfp::lin-5 function, 48 hours before fixation of their embryos. Representative fluorescence microscopy images of embryos stained with anti-LIN-5 (red) and anti-GFP (green) antibodies, and DAPI to visualize DNA. All images taken with same exposure time, objective and magnification. Anterior to the left, scale bars 10 μm.