Fig 6.TIF (8.44 MB)
T168 and T181 mutants show normal subcellular LIN-5 localization.
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posted on 2016-10-06, 17:30 authored by Vincent Portegijs, Lars-Eric Fielmich, Matilde Galli, Ruben Schmidt, Javier Muñoz, Tim van Mourik, Anna Akhmanova, Albert J. R. Heck, Mike Boxem, Sander van den HeuvelImmunohistochemical staining of embryos heterozygous for egfp::lin-5 and wild type or phosphomutant lin-5 as indicated. L4 animals were treated with feeding RNAi against control or egfp RNAi to specifically remove egfp::lin-5 function, 48 hours before fixation of their embryos. Representative fluorescence microscopy images of embryos stained with anti-LIN-5 (red) and anti-GFP (green) antibodies, and DAPI to visualize DNA. All images taken with same exposure time, objective and magnification. Anterior to the left, scale bars 10 μm.
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CDK 1-cyclin BControls Mitotic Spindle Positioningpolarity kinase PKC -3 phosphorylates LINcasein kinase 1.GSK 3 phosphorylationvivo phosphorylated LINGPRresidues form partacid binding sitemitotic spindle segregatesvivo phosphorylated residuescyclin-dependent kinase consensus sitechromosome segregationCRISPR
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