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pone.0223974.g006.tif (4.33 MB)

Subcellular localization analyses of PmCFAT1 and PmCFAT2.

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posted on 2019-10-16, 20:07 authored by Tengxun Zhang, Tingting Huo, Anqi Ding, Ruijie Hao, Jia Wang, Tangren Cheng, Fei Bao, Qixiang Zhang

(A) Prediction of protein subcellular localization of PmCFATs with WoLF PSORT Server. Note: nucl represents nucleus; cyto represents cytoplasm; mito represents mitochondria; plas represents plasma membrane; chlo represents chloroplast. (B) Restriction enzyme digestion of pSuper1300-GFP-PmCFATs. M: DL 15,000 Marker; 1 and 3 represent the enzyme digestion products of pSuper1300::PmCFAT1::GFP and pSuper1300::PmCFAT2::GFP, respectively; 2 and 4 represent circular plasmids of pSuper1300::PmCFAT1::GFP and pSuper1300::PmCFAT2::GFP, respectively, as controls. (C) Subcellular localization of PmCFAT1 and PmCFAT2. The fusion proteins were observed under a confocal laser scanning microscope. a, e, i show the green fluorescence channel; b, f, j show the chloroplast autofluorescence channel. c, g and k show the bright field channel; d, h and l were created from the images shown in the first two panels. a-d: bar = 10 μm; e-l: bar = 15 μm.

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