Fluorescence emission spectra of native and denatured HoLaMa.

(A) Solutions containing 500 nM HoLaMa (in 50 mM sodium phosphate, 50 mM NaCl, pH 8.0) and supplemented (blue line) or not (red line) with 7.6 M urea were excited at 280 nm. The emission spectra were recorded over the 290–400 nm interval and are reported in arbitrary units (a.u.). (B) Difference emission spectrum between native and denatured HoLaMa.

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