Sprint is essential for the starvation-sensitivity phenotype of <i>vap</i>.

<p>The genetic relationship between <i>vap</i> and <i>spri</i> mutants was evaluated using the starvation-sensitivity set up as in <b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0209759#pone.0209759.g005" target="_blank">Fig 5</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0209759#pone.0209759.s004" target="_blank">S4 Fig</a></b>. Females of indicated genotypes were used to record for survival upon complete nutritional deprivation at 25°C. The strong sensitivity to starvation of <i>vap</i><sup><i>2</i></sup> mutants is suppressed when combined to the <i>spri</i><sup><i>6G1</i></sup>-null mutants (white arrow). Thus, Spri acts after Vap. When in the <i>vap</i> mutant context, <i>spri</i> heterozygous flies (<i>vap-/-</i>, <i>spri-/+</i>) shows midway suppression (grey arrow), and thus dosage effect of <i>spri+</i>. Single mutant <i>spri</i><sup><i>6G1</i></sup> or double mutant <i>vap</i><sup><i>2</i></sup>, <i>spri</i><sup><i>6G1</i></sup> has greater resistance than control. Male genotypes resulted in all the same effects. Genotypes. Control females: <i>w</i><sup><i>1118</i></sup><i>/ w</i><sup><i>1118</i></sup>. <i>vap</i><sup><i>2</i></sup><i>/ vap</i><sup><i>2</i></sup>. <i>spri</i><sup><i>6G1</i></sup><i>/spri</i><sup><i>6G1</i></sup>. Assay females: <i>vap</i><sup><i>2</i></sup>, <i>spri</i><sup><i>6G1</i></sup><i>/ vap</i><sup><i>2</i></sup>, <i>spri</i><sup><i>6G1</i></sup>. <i>vap</i><sup><i>2</i></sup>, <i>spri</i><sup><i>6G1</i></sup><i>/ vap</i><sup><i>2</i></sup>, <i>+</i>.</p>