Southern blot analysis of the external transcribed spacer (ETS) patterns for each sample using total genomic DNA that was cut with the XbaI and DraI restriction enzymes.

Lanes ♀ and ♂ represent the maternal parent (Ascocenda John De Biase ‘Blue’) and the paternal parent (Phalaenopsis Chih Shang's Stripes), respectively. Lanes 1–27 represent the putative F1 hybrids.