pone.0230915.g003.tif (543.96 kB)
Screening of N. cholesterolicum NRRL5767 transformants by colony PCR.
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posted on 2020-03-27, 20:05 authored by Jenq-Kuen Huang, Kadidia Samassekou, Hekmat B. Alhmadi, David R. VanDerway, Joshua D. Diaz, Jacob A. Seiver, Shawn W. McClenahan, Scott M. Holt, Lisa WenLanes 1, 12, and 14: Hi-Lo DNA marker; lanes 2–11: selected apramycin resistant N. cholesterolicum NRRL5767 transformants were the sources of DNA template; lane 13: purified pCRISPomyces-2/sgRNA was used as DNA template; lane 15: purified pCRISPomyces-2 was used as DNA template.
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Nocardia cholesterolicum NRRL 5767. Existencealcohol dehydrogenase geneknockout mutants offer improvementsoleate hydratase isozymesNocardia cholesterolicum NRRL 5767Nocardia cholesterolicum NRRL 5767DNAGolden Gate Assemblyalcohol dehydrogenase activityNocardia cholesterolicum NRRL 5767 strainalcohol dehydrogenase knockout mutants10- hydroxystearic acidoleic acid10- ketostearic acidCRISPRalcohol dehydrogenase
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