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Screening of N. cholesterolicum NRRL5767 transformants by colony PCR.

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posted on 2020-03-27, 20:05 authored by Jenq-Kuen Huang, Kadidia Samassekou, Hekmat B. Alhmadi, David R. VanDerway, Joshua D. Diaz, Jacob A. Seiver, Shawn W. McClenahan, Scott M. Holt, Lisa Wen

Lanes 1, 12, and 14: Hi-Lo DNA marker; lanes 2–11: selected apramycin resistant N. cholesterolicum NRRL5767 transformants were the sources of DNA template; lane 13: purified pCRISPomyces-2/sgRNA was used as DNA template; lane 15: purified pCRISPomyces-2 was used as DNA template.

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