Fig 1.tif (185.61 kB)
Schematic figure and features describing the three different scFv magnetic bead coupling strategies; epoxy, streptavidin and anti-FLAG.
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posted on 2018-02-13, 18:25 authored by Anna Säll, Daniel Corbee, Sara Vikström, Filip Ottosson, Helena Persson, Sofia WaldemarsonThe epoxy format is different to the other two in that scFv are coupled directly to the magnetic beads through a covalent coupling to any lysine residue on the scFv. This results in randomly oriented (non-oriented) scFv on the beads. As opposed to the streptavidin-coated beads and M2 anti-FLAG beads where the scFv is immobilized through coupling to the bead surface through a linker at the back-end of the scFv and thus an oriented coupling that is not interfering with the binding site of the scFv. The steptavidin and anti-FLAG formats also require much less scFv and shorter coupling time.
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immunoaffinity platform AFFIRMbiomarker candidatesmeasurementserumverification studiesbeadsFLAG tagmass spectrometry readoutbiomarker targetspgprotein assaysmultiplexing capacityprotein enrichment11- plex setupanalysis formatsdetection capacityimmunoaffinity assay AFFIRMSRMbiotin-tagged scFv3- plex setupantibody immobilization100 timeschain antibodiesreaction monitoringAFFInity sRManti-FLAG antibody
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