SEC complexation experiments.

Cartoons and SEC profiles of the various IL15 binding proteins and IL15. The individual protein peaks (marked with cartoons) always remained visible and were not combined into a single complex peak. (A) F8IL15Rα binding to muIL15. (B) SEC profile using a F8IL15Rα:muIL15 biolegend ratio of 57:1. (C) SEC profile using a F8IL15Rα:muIL15 peprotech ratio of 2:1. (D) SEC profile using a F8IL15Rα:muIL15 biolegend batch 2 ratio of 0.6:1. (E) muIL15RαFc binding to muIL15. (F) SEC profile using a muIL15RαFc:muIL15 biolegend ratio of 29:1. (G) SEC profile using a muIL15RαFc:muIL15 peprotech ratio of 0.8:1. (H) aIL15mAb binding to muIL15. (I) SEC profile using a aIL15mAb:muIL15 biolegend ratio of 18:1. (J) SEC profile using a aIl15mAb:muIL15 peprotech ratio of 0.2:1. (K) huIL15RαFc binding to huIL15. (L) SEC profile using a huIL15RαFc:huIL15 ratio of 0.3:1.