SEC complexation experiments.

2019-07-26T17:25:07Z (GMT) by Anja Sophie Schmid Dario Neri

Cartoons and SEC profiles of the various IL15 binding proteins and IL15. The individual protein peaks (marked with cartoons) always remained visible and were not combined into a single complex peak. (A) F8IL15Rα binding to muIL15. (B) SEC profile using a F8IL15Rα:muIL15 biolegend ratio of 57:1. (C) SEC profile using a F8IL15Rα:muIL15 peprotech ratio of 2:1. (D) SEC profile using a F8IL15Rα:muIL15 biolegend batch 2 ratio of 0.6:1. (E) muIL15RαFc binding to muIL15. (F) SEC profile using a muIL15RαFc:muIL15 biolegend ratio of 29:1. (G) SEC profile using a muIL15RαFc:muIL15 peprotech ratio of 0.8:1. (H) aIL15mAb binding to muIL15. (I) SEC profile using a aIL15mAb:muIL15 biolegend ratio of 18:1. (J) SEC profile using a aIl15mAb:muIL15 peprotech ratio of 0.2:1. (K) huIL15RαFc binding to huIL15. (L) SEC profile using a huIL15RαFc:huIL15 ratio of 0.3:1.