SEC complexation experimental scheme and positive control.

(A+B) Scheme of the SEC complexation experiments visualizing (A) IL15Rα binding to IL15 by showing (B) the merging of the separate protein peaks into one single complex peak. (C) Positive control experimental scheme of F8IL15Rα binding to its cognate antigen EDA and (D) experimental data showing peak merging at equimolar amounts of individual complex components. EDA = extra-domain A of fibronectin, F8Ab = F8 antibody.