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Ribosome dynamics under experimentally reported initiation and elongation rates.

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posted on 2019-10-16, 17:38 authored by Luis U. Aguilera, William Raymond, Zachary R. Fox, Michael May, Elliot Djokic, Tatsuya Morisaki, Timothy J. Stasevich, Brian Munsky

A) Simulated mean number of codons between ribosomes for the β-actin gene as function of initiation and elongation constants. In the plot, previous literature initiation and elongation values are highlighted by the squares [610], and values estimated in this study are denoted by asterisks. B) Simulated number of collisions per ribosome as a function of initiation and elongation constants. C) Top panel, kymograph showing the ribosomal dynamics for SunTag-24X-Kif18b using experimentally determined parameters ki = 1/100 sec−1 and = 3.1 aa/sec [10]. Center panel, kymograph showing the ribosomal dynamics for FLAG-10X-KDM5B using experimentally determined parameters ki = 1/30 sec−1 and = 10 aa/sec [6]. Bottom panel, kymograph showing the ribosomal dynamics for SunTag-56X-Ki67 using experimentally determined parameters ki = 1/13 sec−1 and = 13.2 aa/sec [9]. White lines in kymographs represent single ribosome positions, and green spots represent ribosome collisions.

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