Results of RT-QuIC analysis.
ThT fluorescence was measured by relative fluorescence units (rfu) with saturation occurring at 65,000 for every 42 min. The RT-QuIC responses were performed using 10−3 dilution of sCJD patient with 129MM type brain homogenate (NHBX0/0001), 10−5 CJD negative control brain homogenate (NHBZ0/0005), 15ul of 10−1 dilution of CSF from 4 gCJD cases with V180I (the Patients nos. 1, 2, 3 and 5) and 1 sCJD case with 129MM. Each point represents the mean of 4 replicate rfu readings.