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Results of RT-QuIC analysis.

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posted on 2016-06-24, 19:25 authored by Sol Moe Lee, Myungguen Chung, Jae Wook Hyeon, Seok Won Jeong, Young Ran Ju, Heebal Kim, Jeongmin Lee, SangYun Kim, Seong Soo A. An, Sung Beom Cho, Yeong Seon Lee, Su Yeon Kim

ThT fluorescence was measured by relative fluorescence units (rfu) with saturation occurring at 65,000 for every 42 min. The RT-QuIC responses were performed using 10−3 dilution of sCJD patient with 129MM type brain homogenate (NHBX0/0001), 10−5 CJD negative control brain homogenate (NHBZ0/0005), 15ul of 10−1 dilution of CSF from 4 gCJD cases with V180I (the Patients nos. 1, 2, 3 and 5) and 1 sCJD case with 129MM. Each point represents the mean of 4 replicate rfu readings.

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