Relative mRNA expression of endocannabinoid signaling-related proteins in the spleen of rats exposed to alcohol during adolescence and control rats grouped by sex.

(A) CB₁ mRNA levels (fold change); (B) CB₂ mRNA levels (fold change); (C) PPARα mRNA levels (fold change); (D) NAPE-PLD mRNA levels (fold change); (E) DGLα mRNA levels (fold change); (F) DGLβ mRNA levels (fold change); (G) FAAH mRNA levels (fold change); (H) MGL mRNA levels (fold change); and (I) FAT/CD36 mRNA levels (fold change). The bars represent means ± SEM. The data were analyzed by two-way ANOVA and significant main effects or interaction between factors was indicated as follows: (*) p<0.05 and (**) p<0.01 denote significant main effect of adolescent alcohol exposure; (^###) p<0.001 denotes significant interaction between adolescent alcohol exposure and sex. (^aaa) p<0.001 denotes significant differences compared to female control rats after post hoc tests; (^bbb) p<0.001 denotes significant differences compared to female alcohol-exposed rats after post hoc tests.