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Relative RNA accumulation of allergen-encoding genes in the pulp and the peel of tomato fruits from the lines IL12-4, IL7-3 and M82.

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posted on 16.05.2016 by Alessandra Ghiani, Nunzio D’Agostino, Sandra Citterio, Assunta Raiola, Riccardo Asero, Amalia Barone, Maria Manuela Rigano

qRT-PCR analyses were carried out with primers pairs for the genes Solyc10g075090 (nsLTP 1), Solyc10g080210 (PG2A), Solyc07g064170 (PME1), Solyc07g064180 (PME2.1) using an elongation factors (Solyc06g005060) as endogenous control. The transcript amount in the pulp of M82 fruit was arbitrarily set to 1 and it was used as calibrator for relative expression levels in each comparison. Asterisks indicate values that are significantly different from M82 pulp (*p < 0.05, ** p < 0.01, *** p < 0.001).