Regulation of GCRs by individual telomere binding proteins.

(A) Schematic representation of the S. pombe shelterin complex. (B) GCR rates of telomere-binding protein mutants. (C) Numbers of different types of GCRs identified in 5-FOA/FUdR-resistant clones derived from strains with the indicated genetic backgrounds. (D) Distribution of GCR (exclusively deletion types) junctions in the breakpoint region. Each arrowhead represents the location of a junction in the 5-FOA/FUdR-resistant clones. (E) GCR rates of poz1Δ and tpz1-I501A/R505E mutants in wild-type, taz1Δ, and rap1Δ backgrounds. Asterisks represent a statistically significant difference (P < 0.05) from the wild-type strain, unless otherwise noted. NS: non-significant (P > 0.05). P-values were determined by the two-tailed Mann–Whitney test.