Reduced liver cell death and enhanced hepatocyte proliferation in med23Δli mice after chronic administration of CCl4.
(A) Representative views of TUNEL staining and α-SMA staining in the liver sections of med23f/f and med23Δli mice. (B) Quantification of TUNEL-positive cells from the liver sections of med23f/f and med23Δli mice (med23f/f, n = 6; med23Δli, n = 7). (C) The expression of antiapoptotic genes (cIAP1 and cIAP2) in whole-liver extracts was analyzed by qRT-PCR (n = 7 per group). (D) Representative views and quantification of PCNA staining in the liver sections of med23f/f and med23Δli mice (n = 7 per group). (E) qRT-PCR analysis of proliferative genes (Hgf, CyclinD1, c-Fos, and c-Jun) in whole-liver extracts of med23f/f and med23Δli mice (n = 7 per group). Data are presented as means ± SEM. *P < 0.05, **P < 0.01. For underlying data, see S1 Data file. α-SMA, alpha-smooth muscle actin; CCl4, carbon tetrachloride; cIAP, cellular inhibitor of apoptosis protein; Hgf, hepatocyte growth factor; med23, Mediator complex subunit 23; med23Δli, liver-specific knockout of Med23; med23f/f, med23-floxed; PCNA, proliferating cell nuclear antigen; qRT-PCR, quantitative real-time PCR.