Recovery of gene expression after long-term heterochromatization.

A) Re-expression of the CpGDep A and CpGDep D cell lines after five weeks of csHP1α-induced silencing, with or without the addition of 5-aza. B) Re-expression of the CpGFull E and CpGFull G cell lines after five weeks of csHP1α-induced silencing, with or without the addition of 5-aza. Dotted line represents baseline expression for each cell line. Flow cytometry was performed in biological triplicate on Attune 1. n = 3, ** P ≤ 0.01. *** P ≤ 0.001.