Fig 2.TIF (187 kB)
Recognition of peptides by salivary IgA.
figure
posted on 2016-08-31, 10:26 authored by Saleh Umair, Qing Deng, Joanna M. Roberts, Richard J. Shaw, Ian A. Sutherland, Anton PernthanerPlates were coated 2 μg/ml of individual peptides or with purified CarLA (2 μg/ml). A CarLA-negative saliva pool was used to test for non-specific binding. Peptides PAB1.C7C-3, -4, 5-, 16-, 18 and 37 were recognised by salivary IgA from an anti-CarLA positive pool. Serial dilutions of saliva pools were used in assays and results from 1:10 dilutions presented. Data are representative of at least 3 independent repeat assays.
