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Real-time PCR analysis of gluconeogenesis mRNA expression in the liver.

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posted on 2014-02-10, 03:23 authored by Michelle L. Van Sinderen, Gregory R. Steinberg, Sebastian B. Jørgensen, Sarah Q. To, Kevin C. Knower, Colin D. Clyne, Jane Honeyman, Jenny D. Chow, Kerrie A. Herridge, Margaret E. E. Jones, Evan R. Simpson, Wah Chin Boon

Real-time-PCR analyses of G6Pase, PEPCK, IRS1, IRS2 and GSK3β mRNA expression were performed on cDNA derived from total RNA prepared from fasted liver tissue of (a) 3 and (b) 6 month-old male wildtype (WT, n = 8), aromatase knockout (KO, n = 8) and 2.5 µg/day 17β-estradiol-treated KO (KOE, n = 7) mice. Expression data from 7–8 samples per genotype are shown following normalization for cyclophilin mRNA expression, and presented from replicate analysis as the mean ± SD. *p<0.05,**p<0.01 and ***p<0.001 versus expression in age-matched WT samples and ##p<0.01, ###p<0.05 versus KO samples.

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