Real-time PCR analysis of gluconeogenesis mRNA expression in the liver.

Real-time-PCR analyses of G6Pase, PEPCK, IRS1, IRS2 and GSK3β mRNA expression were performed on cDNA derived from total RNA prepared from fasted liver tissue of (a) 3 and (b) 6 month-old male wildtype (WT, n = 8), aromatase knockout (KO, n = 8) and 2.5 µg/day 17β-estradiol-treated KO (KOE, n = 7) mice. Expression data from 7–8 samples per genotype are shown following normalization for cyclophilin mRNA expression, and presented from replicate analysis as the mean ± SD. *p<0.05,**p<0.01 and ***p<0.001 versus expression in age-matched WT samples and ##p<0.01, ###p<0.05 versus KO samples.