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Raloxifene inhibits osteoclastic gene expression in vitro.

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posted on 2016-11-01, 17:39 authored by Mayu Morita, Yuiko Sato, Ryotaro Iwasaki, Tami Kobayashi, Ryuichi Watanabe, Takatsugu Oike, Kana Miyamoto, Yoshiaki Toyama, Morio Matsumoto, Masaya Nakamura, Hiromasa Kawana, Taneaki Nakagawa, Takeshi Miyamoto

Osteoclast progenitors from wild-type mice were cultured with or without raloxifene (RAL, 1μM) in the presence or absence of M-CSF 50ng/ml (M) and RANKL 25ng/ml (R). Osteoclast formation was evaluated by TRAP staining (A), by the number of multi-nuclear TRAP-positive cells (B) and by Cathepsin K, NFATc1, DC-STAMP expression as analyzed by realtime PCR (C). Data represent mean Cathepsin K, NFATc1 or DC-STAMP expression relative to β-actin ± SD (n = 3). Bar = 100 μm. *P<0.05; **P<0.01; NS, not significant (unpaired two-tailed Student’s t-test). Representative data of at least three independent experiments are shown.

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