RT-qPCR assays of PKS8-1 cluster genes in overexpression transformants of the aflR-like gene and vector controls relative to wild-type expression in culture.
The wild type, two transformants of vector control (VC-1, VC-2), and two transformants of the aflR-like transcription factor overexpression (OX-4, OX-8) were grown in PDB medium. RT-qPCR assays were performed on these samples for the PKS8-1 cluster genes. Fold-change gene expression is shown on a log scale relative to wild type set to 1. Error bars indicate standard error from six biological replicates. Dotted bar: wild type; hatched bars: vector controls; black bars: overexpression strains.