RT-PCR analysis results are affected by sampling system, RNA extraction method and the choice of reference gene.
SIGLEC-7 gene expression profiles of Series B with additional DNAse digestion. Blood was collected from 12 donors; triplicates from each donor were reverse transcribed and experimental duplicates from each sample run in Real-Time PCR reactions. Data were normalized to (A) PPIA, (B) β-ACTIN, (C) β-TUBULIN and (D) to a combination of these 3 reference genes. Results are shown as relative to the randomly chosen subject #1. A-C show means of experimental duplicates ± SE, D shows means of pooled triplicates from each donor.