pone.0202307.g004.tif (833.42 kB)
Quantitative real-time PCR (qPCR) against human specific sequence.
figure
posted on 2018-08-16, 17:47 authored by Jeong-Seob Won, Hyun Nam, Hye Won Lee, Ji-Yoon Hwang, Yu-Jeong Noh, Do-Hyun Nam, Sun-Ho Lee, Kyeung Min JooTo detect U87MGs in the CNS of rats with SCI, each region of the CNS was analyzed by qPCR using human-specific primers. (A) Relative amounts of human Alu sequence were quantified and compared. Height = Average, Error bar = Standard deviation. *, P < 0.05. P.C. = positive control (U87MG cells). (B) Specificity of qPCR was confirmed by the size of PCR product (124 base pairs).
