Quantitative real-time PCR (qPCR) against human specific sequence.

To detect U87MGs in the CNS of rats with SCI, each region of the CNS was analyzed by qPCR using human-specific primers. (A) Relative amounts of human Alu sequence were quantified and compared. Height = Average, Error bar = Standard deviation. *, P < 0.05. P.C. = positive control (U87MG cells). (B) Specificity of qPCR was confirmed by the size of PCR product (124 base pairs).