Fig 4.tif (307.4 kB)
Quantification of BAL fluid chemokine levels by protein immunochemistry.
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posted on 2018-04-12, 17:36 authored by Jungnam Lee, Ivan Arisi, Ermanno Puxeddu, Lazarus K. Mramba, Massimo Amicosante, Carmen M. Swaisgood, Marco Pallante, Mark L. Brantly, C. Magnus Sköld, Cesare SaltiniChemokines were measured in un-concentrated BAL fluid by protein immunochemistry on IPF affected individuals (closed circles) and Healthy Controls (open circles). CXCL-5 was quantified using the Chemokine Human 5-Plex Panel II for the Luminex 100/200 platform. PPBP/CXCL-7, CXCL-10, CCL13 and CCL18 were measured using enzyme-linked immunosorbent assay (ELISA) kits. Mann-Whitney test (*) and (**) denote statistical significance (P < 0.05, P < 0.01, respectively) vs. control.
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p-valueangiogenic activation patternmacrophage activationfibrosisBAL cellsGene expression analysisCxclRNAalveolar epithelial damageCTRmacrophage iron accumulation IdiopathicKEGGferric ammonium citrateiron-dependent ROS generationdata show alveolar macrophage expressionMH-Salveolar macrophage activation phenotypeupregulated M 1-typeMTPPIPF patients
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