Protein for both PAR1 and Par3 is undetectable in platelets from hPAR1-KI mice.
Whole cell lysates of platelets isolated from hPAR1-KI mice, wild type mice, or humans were probed for either PAR1 or Par3 protein by Western blot. PAR1 was readily detected in human platelets but not in platelets from wild type or hPAR1-KI mice. In the same samples, Par3 was detected in platelets from wild type mice but not in platelets from either hPAR1-KI mice or humans. Actin was used as a protein loading control for all lysates. Blots shown are representative of n = 3 experiments.