Profiling of cell surface receptor phenotype of VZV cultured NK cells.
PBMCs were mock cultured, exposed to VZV, or VZV infected untreated or with 200 U/ml IL-2 for 1 day. NK cells (viable CD3–CD56+ cells) were assessed by flow cytometry for cell surface receptor expression (A), and heatmaps show receptor expression as measured by percentage positive with hierarchical clustering for 3 donors (denoted A, B and C) (B). (C) Graph shows fold change over mock in median fluorescence intensity (MFI) for ubiquitously expressed receptors (n = 3). Symbols represent individual donors. Dotted line at y = 1 indicates point of variance from mock. Statistical analysis performed compared to mock. **P < 0.01, ***P < 0.001, ****P < 0.0001 (repeated measures two-way ANOVA with Dunnett’s correction).