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Principal component analysis for 96.96 Dynamic Array including all tissues datasets.

2019-12-03T18:44:22Z (GMT) by Dominika Borowska Richard Kuo Richard A. Bailey Kellie A. Watson Pete Kaiser Lonneke Vervelde Mark P. Stevens

Analysis indicates broad similarities and differences in transcription of immune-related genes in bursa, spleen, ileum and caecal tonsils and peripheral blood leukocytes (PBLs); data points represent individual samples of sibling-test (filled markers) and pedigree farms (no fill markers).

Categories

  • Genetics
  • Molecular Biology
  • Biotechnology
  • Immunology
  • Biological Sciences not elsewhere classified
  • Developmental Biology
  • Infectious Diseases

Keyword(s)

multiplexed qPCR 96.96 Fluidigm Dynamic Array 6 nanolitre reactions broiler chickens PCR-based platform microfluidic system 96 transcripts transcript levels transcriptome data amplicon sequencing chicken macrophages RNA-seq analysis preamplification steps Conventional qPCR analysis Gut-associated lymphoid tissues blood cells transcriptional response 89 genes immune-related genes biosecurity levels multiplexed qPCR-based platform screen vaccine responses chicken immune-related genes multiplex qPCR array 96 samples analyse high-throughput assays blood leukocytes curve analysis pathogen-specific probes dendritic cells

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CC BY 4.0

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