Phylogenetic analysis of PKS8-1.

<p>RAxML was used to create a maximum likelihood phylogenetic tree of PKS protein sequences, using the PKS sequences described previously [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0212229#pone.0212229.ref018" target="_blank">18</a>] and others including the monodictyphenone-producing MdpG from <i>A</i>. <i>nidulans</i>, the cladofulvin-producing ClaG from <i>C</i>. <i>fulvum</i>, and the PKS8-1 homolog sequences identified from <i>P</i>. <i>musae</i> and <i>P</i>. <i>eumusae</i>. The clade containing PKS8-1, its <i>P</i>. <i>musae</i> and <i>P</i>. <i>eumusae</i> homologs, the monodictyphenone-producing PKS from <i>A</i>. <i>nidulans</i>, and the cladofulvin-producing PKS from <i>C</i>. <i>fulvum</i> is shown with a red box. Bootstrap values are indicated on the tree, and the scale bar indicates substitutions per site. <i>An</i> = <i>Aspergillus nidulans</i>; <i>Ap</i> = <i>Aspergillus parasiticus</i>; <i>As</i> = <i>Alternaria solani</i>; <i>At</i> = <i>Aspergillus terreus</i>; <i>Bm</i> = <i>Bipolaris maydis</i>; <i>Bo</i> = <i>Bipolaris oryzae</i>; <i>Cf</i> = <i>Cladosporium fulvum</i>; <i>Cg</i> = <i>Colletotrichum graminicola</i>; <i>Cn</i> = <i>Cercospora nicotianae</i>; <i>Czm</i> = <i>Cercospora zeae-maydis</i>; <i>Ed</i> = <i>Exophiala dermatitidis</i>; <i>Fm</i> = <i>Fusarium moniliforme</i>; <i>Fv</i> = <i>Fusarium verticillioides</i>; <i>Gl</i> = <i>Glarea lozoyensis</i>; <i>Pc</i> = <i>Penicillium citrinum</i>; <i>Pg</i> = <i>Penicillium griseofulvum</i>; <i>Pe</i> = <i>Pseudocercospora eumusae</i>; <i>Pf</i> = <i>Pseudocercospora fijiensis</i>; <i>Pm</i> = <i>Pseudocercospora musae</i>.</p>